Electrochemical Flow Enzyme Immunoassay by Means of a Needle-Shaped Sampler/Reactor.
نویسندگان
چکیده
منابع مشابه
Electrochemical enzyme-immunoassay
"Immunoassay" defines the body of techniques which use the antibody (Ab) macromolecule, usually of the IgG class, for the detection and quantitation of an enormous range of simple and complex antigen (Ag) molecules. The success of the methods relies on both the specificity and formation constant of the Ab used, and the ability to detect the interaction between Ab and Ag. Sensitive assays in com...
متن کاملElectrochemical enzyme immunoassay for phenytoin by flow-injection analysis incorporating a redox coupling agent.
Using phenytoin as a model analyte, we demonstrate an electrochemical enzyme immunoassay based on flow-injection analysis and incorporating 2,6-dichloroindophenol (DCIP) as a redox coupling agent. DCIP reacts with NADH to form NAD+ and DCIPH2, the reduced form of the coupling agent. The production of DCIPH2 is monitored at +250 mV vs Ag/AgCl. This low applied potential improves selectivity in t...
متن کاملCompetitive heterogeneous enzyme immunoassay for theophylline by flow-injection analysis with electrochemical detection of p-aminophenol.
A competitive enzyme-linked immunoabsorbent assay based on the flow-injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated duri...
متن کاملDETERMINATION OF SERUM CORTISOL LEVEL BY A DIREC T ENZYME IMMUNOASSAY USING PENICILLINASE AS LABEL
Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...
متن کاملNoncompetitive enzyme immunoassay for carcinoembryonic antigen by flow injection chemiluminescence.
BACKGROUND Recently, many automated immunoassay analyzers have been developed for carcinoembryonic antigen (CEA) to overcome the shortcomings in traditional immunoassay methods that are time-consuming and labor-intensive. Flow injection immunoassay (FIIA) has been increasingly applied to laboratory medicine due to its ease in automation, rapid speed and reproducible results. It is important to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2003
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.19.155